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ninta agarose resin  (Qiagen)


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    Structured Review

    Qiagen ninta agarose resin
    Ninta Agarose Resin, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 28436 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ninta+resin/Ni-NTA+Agarose/pm42020439-201-5-8
    Average 99 stars, based on 28436 article reviews
    ninta agarose resin - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Incubation:

    Article Title: Discovery of a distinct type of methylenetetrahydrofolate reductase family that couples with tetrahydrofolate-dependent demethylases.
    Article Snippet: The supernatans was then collected and filtrated using a 0.22 μm filter (Millipore, Burlington, MA, USA). .. The filtered supernatant was mixed with NiNTA resin (Qiagen,Hilden, Germany) and incubated on a rotary shaker for 2 h at 4 °C. .. The supernatant was centrifuged at 20,000 rpm for 20mins, resuspended with wash buffer [50mM Tris-HCl (pH 7.4), 500mM NaCl, 1mM dithiothreitol (DTT), and 100mM imidazole (pH 7.4)], and loaded onto an Econo-Column (Bio-Rad Laboratories, Hercules, CA, USA) filled withNi-NTA resin.

    Article Title: Telomeres stall DNA loop extrusion by condensin.
    Article Snippet: .. Extract was cleared by centrifugation at 186,000g for 1 hour at 4 ◦ C and then incubated at 4 ◦ C with NiNTA resin (QIAGEN) for 4 h. Mixture was poured into an Econo-Column®Chromatography column (BIO-RAD). ..

    Purification:

    Article Title: Supporting Information In-Plate Chemical Synthesis of Isopeptide-Linked SUMOylated Peptide Fluorescence Polarization Reagents for High-Throughput Screening of SENP Preferences
    Article Snippet: Lysis was performed using a sonicator 3000; misonix for 3 minutes with 15-/45-s on/off cycles followed by centrifugation at 24,000 x g for 45 minutes at 4oC. .. The proteins were purified by his-tag purification using Qiagen NiNTA resin. ..

    Article Title: Supporting Information for H3K14ac facilitates the reinstallation of constitutive heterochromatin in Drosophila early embryos by engaging Eggless/SetDB1
    Article Snippet: .. After sonication at 4°C, the supernatant was collected by centrifugation at 10000 rpm for 1 hour and further purified using NiNTA resin (Qiagen). ..

    Centrifugation:

    Article Title: Binding of the brain G protein G⍺ o to its potential effector RASA3 is promoted by Ca 2 .
    Article Snippet: .. After centrifugation, the supernatant, containing cleaved G⍺o protein, was mixed with 100 μL of packed NiNTA resin (Qiagen, 30210) to remove the His-TEV and any GST-His6 or full-length GST6His-TEV-G⍺o or present. .. For most experiments, the G⍺o protein in solution was Jo urn al Pr -pr oo f concentrated to 0.5 mL using a Pierce protein concentrator (PES, 10K MWCO) and run over a Superdex 200 Increase 10/300 GL size exclusion column (Cytiva) using HED buffer not containing any nucleotide to isolate monodispersed G⍺o and remove free GDP.

    Article Title: Telomeres stall DNA loop extrusion by condensin.
    Article Snippet: .. Extract was cleared by centrifugation at 186,000g for 1 hour at 4 ◦ C and then incubated at 4 ◦ C with NiNTA resin (QIAGEN) for 4 h. Mixture was poured into an Econo-Column®Chromatography column (BIO-RAD). ..

    Article Title: Supporting Information for H3K14ac facilitates the reinstallation of constitutive heterochromatin in Drosophila early embryos by engaging Eggless/SetDB1
    Article Snippet: .. After sonication at 4°C, the supernatant was collected by centrifugation at 10000 rpm for 1 hour and further purified using NiNTA resin (Qiagen). ..

    other:

    Article Title: Telomeres stall DNA loop extrusion by condensin.
    Article Snippet: The fragments were ligated together by using a T4 DNA ligase in T4 ligase buffer (New England Biolabs), with 1 mM ATP overnight at 16 o C. The final constructs were transformed into E. coli NEB 10-beta cells (New England Biolabs) and all constructs were sequence verified using plasmidsaurus Oxford Nanopore long read sequencing.

    Article Title: TaFT-D1 positively regulates grain weight by acting as a coactivator of TaFDL2 in wheat.
    Article Snippet: Tissue sections (4 lm) were cut on a microtome (Leica Microsystems, Nussloch, Germany) and stained with toluidine blue.

    Sonication:

    Article Title: Supporting Information for H3K14ac facilitates the reinstallation of constitutive heterochromatin in Drosophila early embryos by engaging Eggless/SetDB1
    Article Snippet: .. After sonication at 4°C, the supernatant was collected by centrifugation at 10000 rpm for 1 hour and further purified using NiNTA resin (Qiagen). ..



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